Quantification of lipid in dysferlin-deficient muscles and other dystrophies
This project builds upon the high levels of fat reported in the most severely affect muscles in dysferlin-deficient A/J mice in our recent paper (Terrill et al, 2013, FEBS J. Jan 19. [Epub]). The major aim of this research is to develop a quantitative histological technique to describe the extent and nature of this fat in mouse and human dysferlin-deficient muscles, in comparison with other muscular dystrophies. In addition, preliminary molecular studies will profile the expression of key adipogenic and lipogenic genes in a range of dysferlin-deficient BLAJ mouse muscles at different ages.