Membrane Repair Assays

Paul McNeil, PhD

Medical College of Georgia (Augusta, GA)

Dr. McNeil is a Professor in the Department of Cellular Biology and Anatomy at the Institute of Molecular Medicine and Genetics, Medical College of Georgia (Augusta, GA).

Past Projects

To probe the molecular mechanism of muscle cell membrane repair and to develop novel high-throughput assays that can be used in the discovery of small chemicals capable of repair facilitation.

This application has 3 major aims, all of which arise from our ongoing research aimed at understanding membrane repair in general, and limb-girdle type IIB muscular dystrophy and Myoshi myopathy (LGMD2B/MM) in particular. 1. Test the role of annexins, and other protein candidates, in membrane repair by skeletal muscle cells and the effect of dysferlinopathy on annexin-related and other repair pathways. 2. Develop techniques for quantitatively monitoring membrane repair that can be used in a high-throughput assay capable of detecting small chemicals having the potential for promoting membrane repair by dysferlin null muscle fibers. 3. Continue to act as a community resource, providing advice and help in developing membrane repair assays, and conducting laser-based assays of membrane repair.