Membrane Repair Assays

Paul McNeil, PhD

Medical College of Georgia (Augusta, GA)

Dr. McNeil is a Professor in the Department of Cellular Biology and Anatomy at the Institute of Molecular Medicine and Genetics, Medical College of Georgia (Augusta, GA).

Past Projects

Objective: 
To probe the molecular mechanism of muscle cell membrane repair and to develop novel high-throughput assays that can be used in the discovery of small chemicals capable of repair facilitation.

This application has 3 major aims, all of which arise from our ongoing research aimed at understanding membrane repair in general, and limb-girdle type IIB muscular dystrophy and Myoshi myopathy (LGMD2B/MM) in particular. 1. Test the role of annexins, and other protein candidates, in membrane repair by skeletal muscle cells and the effect of dysferlinopathy on annexin-related and other repair pathways. 2. Develop techniques for quantitatively monitoring membrane repair that can be used in a high-throughput assay capable of detecting small chemicals having the potential for promoting membrane repair by dysferlin null muscle fibers. 3. Continue to act as a community resource, providing advice and help in developing membrane repair assays, and conducting laser-based assays of membrane repair.