Multipotent Adult Progenitor Cells (MAPCs)

Morayma Reyes, MD, PhD

University of Washington (Seattle, WA)

Dr. Reyes is an Assistant Professor in the Department of Pathology and Laboratory Medicine, University of Washington (Seattle, WA).

Past Projects

Objective: 
Explore the use of Muscle Adult Progenitor Cells to treat dysferlin deficiency

Gene therapy and cell therapy are two possible approaches to achieve dysferlin replacement. I propose to use adult stem cells as cell therapy for dysferlinopathies. We have recently characterized a perivascular stem cell population from the skeletal muscle, termed muscle Multipotent Adult Progenitor Cells (MAPCs). Muscle MAPCs transplanted intramuscularly into mdx mice, a mouse model for muscular dystrophy, significantly contribute to muscle regeneration. Thus, MAPCs as a stem cell source may offer many advantages over satellite cells: can be easily isolated, can be massively expanded in vitro, can be easily transduced, can be intravenously and intra-arterially transplanted achieving broad biodistribution and most importantly can be isolated from allogeneic and autologous sources. We propose to first characterize muscle stem cells from the A/J dysferlin deficient mouse model. Then, we propose to intra-arterially transplant wt MAPCs into A/J mice. This will help us determine the biodistribution and early engraftment of MAPCs into multiple muscle tissues. Finally, in order to induce long-term immune tolerance of transplanted MAPC cells, we will co-transplant of hematopoietic stem cells and MAPCs derived from the same donor into A/J mice. If successful this transplantation protocol will be studied in bigger animal models.