Production and Characterization of Chicken-raised Dysferlin Isoform-specific Antibodies

John Robinson, PhD

Ohio State University (Columbus, OH)

Dr. Robinson is a Professor in the Department of Physiology and Cell Biology at the Ohio State University Medical Center (Columbus, OH).

Past Projects

Objective: 
Use chickens to raise antibodies targeted to specific regions of the dysferlin protein, allowing to distinguish between isoforms

The chicken, as a source for antibodies, has several attributes that make it attractive. Most mammalian gene products are recognized as being very foreign and a robust immune response is mounted. This is thought to be related to the evolutionary distance between chicken and say humans in comparison to the distance between humans and rabbits or mice (Larsson et al., 1993). Another important feature of chickens as producers of Abs is the fact that large quantities of antibodies are produced. The specific aims of this project are 1) To design, synthesize, and utilize peptides for the immunization of chickens (hens) to generate isoform-specific antibodies to DYSF; 2) To collect and purify peptide-specific IgY antibodies to DYSF isoforms; and 3) To characterize the DYSF isoform-specific antibodies.