Vesicle Characterization, Secretion and Proteomics
This project examines the role of dysferlin in the trafficking and secretion of different classes of vesicles in differentiated myotubes compared to the immature myoblast precursor cells. The hypothesis is that dysferlin may be critical for the release of a specific class of vesicles that promote either unidirectional or reciprocal signaling between the myoblast and the mature myotube. This signaling may be important for the recruitment of differentiating myoblasts into muscle fibers as a mechanism of general maintenance or repair following injury or disease related degeneration. Understanding the signaling mediated by vesicular trafficking and secretion, and dysferlin’s role in the process, may provide further information about how to stimulate muscle precursor cells to incorporate into and repair damaged or degenerating muscle. In order to characterize the vesicular mediated signaling, SILAC based mass-spectrometry approaches are being employed to characterize the proteomic profile of the different classes of secreted vesicles in wild-type (normal) and dysferlin null cells.
Although the Jain Foundation is no longer financially supporting this project, Dr. Butler-Browne is continuing this line of research under funding from other sources. The Jain Foundation is still interested in the outcome of these studies, and Dr. Butler-Browne continues to update us with regard to the status of the research.