In Vitro High Throughput Screening Assay

Kanneboyina Nagaraju, DVM, PhD

Children's National Medical Center (Washington, DC)

Dr. Nagaraju is an associate Professor of Pediatrics and the director of the Murine Drug testing facility at the CNMC (Washington DC).

Past Projects

Objective: 
Develop an assay system to test drugs for muscle membrane repair

Plasma membrane disruption and resealing are common events in several cell types including skeletal muscle. It is known that disruptions in the plasma membrane in dysferlin deficient muscle are due to defects in the plasma membrane repair. Currently there are no therapies for dysferlin deficient patients. Identification of drugs/compounds that facilitate membrane repair in muscle may not only help in reducing cell damage and dysfunction but also improve muscle functions in dysferlin deficient patients. This project will develop a robust, reproducible plasma membrane repair assay that is amenable for automated analysis to test drugs that facilitate membrane repair in dysferlin-deficient muscle cells.