Biochemical and Therapeutic Characterization of Dysferlin

Kevin Sonnemann, PhD

University of Wisconsin-Madison (Madison, WI)

Dr. Sonnemann is an associate professor in the laboratory of Dr. William Bement in the Department of Zoology at the University of Wisconsin-Madison (Madison, WI).

Research Projects

Objective: 
Characterize and evaluate the therapeutic potential of recombinant membrane repair proteins.

We express and purify recombinant dysferlin proteins and are using an assortment of biochemical, biophysical, and microscopy assays to better understand how dysferlin regulates membrane structure and membrane fusion.  The aims of this project are to 1) investigate the therapeutic potential of candidate small molecules by assessing their ability to restore function to recombinant dysferlin proteins harboring disease-causing mutations, and 2) investigate how alternative splicing of the dysferlin transcript affects the structure and lipid-binding activities of the resultant protein.