Creating Libraries of Nanobodies That Recognize Selected Regions of Dysferlin

Brian Chait, D.Phil and Michael Rout, PhD

Rockefeller University

Brian Chait, D.Phil., is the Camille and Henry Dreyfus Professor at the Rockefeller University in New York and head of the Laboratory for Mass Spectrometry and Gaseous Ion Chemistry. Michael Rout, Ph.D., is also a Professor at the Rockefeller University and head of the Laboratory of Cellular and Structural Biology.

Research Projects

Objective: 
To generate libraries of nanobodies that recognize selected regions of dysferlin

Brian Chait, D.Phil., is the Camille and Henry Dreyfus Professor at the Rockefeller University in New York and head of the Laboratory for Mass Spectrometry and Gaseous Ion Chemistry. He specializes in the development and use of mass spectrometry as a tool for investigating a variety of biological and biochemical phenomena.

Michael Rout, Ph.D., is also a Professor at the Rockefeller University and head of the Laboratory of Cellular and Structural Biology. He uses biochemical, biophysical, and structural approaches to characterize macromolecular assemblies, with an emphasis on the nuclear pore complex, a key part of the pathway that relays information between the nucleus and cytoplasm. His goal is to further develop proteomic technologies that will enable the community to assemble detailed, dynamic representations of the interactions in the cell.

Together, we have recently developed a revolutionary new strategy for recombinant antibody production. Our approach allows the rapid identification and large-scale production of extensive repertoires of bacterially-expressed, high affinity nanobodies against any desired antigen. We are therefore making libraries of nanobodies that recognize selected regions of dysferlin, to be used as diagnostics tools and as basic affinity reagents to explore the dynamic intracellular interaction networks formed by both normal dysferlin and disease-causing mutants.