Dysferlin cDNA Clones

Four full length dysferlin cDNAs have been deposited to Addgene by the Jain Foundation and are available through that site (see below). 

  1. Human dysferlin - original   (accession #NM_003494*)
    View plasmid map and sequence information.
  2. Human dysferlin - v1   (accession #DQ267935)
    Exon 1 variant of Human dysferlin.
    View plasmid map and sequence information.
  3. Mouse dysferlin - transcript variant 2   (accession #NM_001077694)
    Similar to Human dysferlin - original.
    View plasmid map and sequence information.
  4. Mouse dysferlin - transcript variant 1   (accession #NM_021469)
    Similar to Human dysferlin - v1.
    View plasmid map and sequence information.

All 4 cDNAs are provided in the pDONR221 plasmid, which can be used as an entry vector for Invitrogen’s gateway system.  There are also several restriction sites available for traditional cloning.  Please view the example restriction digest confirming the identity of the plasmids. Additional information about the known dysferlin splice variants is also available.

*Note: NCBI Reference Sequence NM_003494.2 has been updated to NM_003494.3 since the creation of these plasmids. There is a one nucleotide base pair change between the two sequences, but the protein sequence is identical.

 

All of these plasmids are available from Addgene.

Link to Plavi Mittal Lab Plasmids