Human dysferlin missense mutation and deletion constructs
Human Dysferlin missense mutation constructs: 115 human cDNA constructs with individual DYSF missense mutations. Developed by Dr. Mohan Viswanathan and Dr. Lenny Guarente. Information about availabe missense mutations can be obtained from the Jain Foundation
Human Dysferlin deletion constructs:
Plasmids containing human dysferlin with every single exon that can be skipped without disrupting the open reading frame are freely available from the Jain Foundation. These human dysferlin deletion constructs are in the multiple cloning site of pUC57-Kan and can be easily cloned into expression vector for analysis. All constructs were made using the full-length original human sequence which contains exons 1 and 17, but does not include 1a, 5a, or 40a (accession #NM_003494).
Deletion construct Requests:
All of the deletion plasmids are available from Addgene.
Download sequence information:
When you click on the links below, you will download a zipped folder containing 4 files including: 1) A pdf with the vector map, full length sequence information, locations of unique restriction sites, and the location of the deletion, 2) the full sequence of the plasmid and insert in a word document, 3) the full sequence of the plasmid and insert in a fasta file and 4) a fully annotated sequence file that can be opened using DNAStar Lasergene software.
- Full-length human dysferlin - original in pUC57-Kan (accession #NM_003494)
Download plasmid map and sequence information. - Exon 7 deletion
Download plasmid map and sequence information. - Exon 8 deletion
Download plasmid map and sequence information. - Exon 9 deletion
Download plasmid map and sequence information. - Exon 14 deletion
Download plasmid map and sequence information. - Exon 17 deletion
Download plasmid map and sequence information. - Exon 19 deletion
Download plasmid map and sequence information. - Exon 24 deletion
Download plasmid map and sequence information. - Exon 25 deletion
Download plasmid map and sequence information. - Exon 30 deletion
Download plasmid map and sequence information. - Exon 32 deletion
Download plasmid map and sequence information. - Exon 34 deletion
Download plasmid map and sequence information. - Exon 35 deletion
Download plasmid map and sequence information. - Exon 36 deletion
Download plasmid map and sequence information. - Exon 37 deletion
Download plasmid map and sequence information. - Exon 38 deletion
Download plasmid map and sequence information. - Exon 41 deletion
Download plasmid map and sequence information. - Exon 42 deletion
Download plasmid map and sequence information. - Exon 43 deletion
Download plasmid map and sequence information. - Exon 45 deletion
Download plasmid map and sequence information. - Exon 49 deletion
Download plasmid map and sequence information.