Human dysferlin deletion constructs

Plasmids containing human dysferlin with every single exon that can be skipped without disrupting the open reading frame are freely available from the Jain Foundation. These human dysferlin deletion constructs are in the multiple cloning site of pUC57-Kan and can be easily cloned into expression vector for analysis. All constructs were made using the full-length original human sequence which contains exons 1 and 17, but does not include 1a, 5a, or 40a (accession #NM_003494).

Requests:
All of these plasmids are also available from Addgene.

Link to Plavi Mittal Lab Plasmids

 

Download sequence information:
When you click on the links below, you will download a zipped folder containing 4 files including: 1) A pdf with the vector map, full length sequence information, locations of unique restriction sites, and the location of the deletion, 2) the full sequence of the plasmid and insert in a word document, 3) the full sequence of the plasmid and insert in a fasta file and 4) a fully annotated sequence file that can be opened using DNAStar Lasergene software.

    1. Full-length human dysferlin - original in pUC57-Kan   (accession #NM_003494)
      Download plasmid map and sequence information.
    2. Exon 7 deletion 
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    3. Exon 8 deletion
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    4. Exon 9 deletion 
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    5. Exon 14 deletion 
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    6. Exon 17 deletion
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    7. Exon 19 deletion 
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    8. Exon 24 deletion 
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    9. Exon 25 deletion
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    10. Exon 30 deletion 
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    11. Exon 32 deletion
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    12. Exon 34 deletion 
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    13. Exon 35 deletion 
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    14. Exon 36 deletion
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    15. Exon 37 deletion 
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    16. Exon 38 deletion 
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    17. Exon 41 deletion
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    18. Exon 42 deletion 
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    19. Exon 43 deletion
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    20. Exon 45 deletion 
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    21. Exon 49 deletion 
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Download all plasmid maps and sequence information.