Mouse Models: B6.Cg-Tg(Ckm-DYSF)3Cam/J

B6.Cg-Tg(Ckm-DYSF)3Cam/J

Availability:
The Jackson Laboratory
Stock number: 014146
Strain information: http://jaxmice.jax.org/strain/014146.html
Phone: 800-422-MICE (800-422-6423)
610 Main Street, Bar Harbor, Maine 04609 USA

Description:
Expression of human DYSF (dysferlin) cDNA is driven by the mouse Ckm (creatine kinase, muscle; MCK) promoter in this transgenic strain. Hemizygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Expression of dysferlin in skeletal muscle is approximately 4-8 fold that of the endogenous protein level. There is no detectable muscle pathology up to one year of age.

Development:
The human DYSF (dysferlin) cDNA with SV40 polyA was inserted downstream of the mouse Ckm (creatine kinase, muscle; MCK) promoter. The transgenic vector was introduced to B6SJLF1 hybrid embryos to create the mutation. This strain was backcrossed to C57BL/6 for six generations by the donating laboratory.

Phenotype:
No phenotype has been published.

Control strain(s):
C57BL/6

References:
Campbell K. 2011. Direct Data Submission to Jackson Laboratories 2010/12/22_y4VaX3 MGI Direct Data Submission :.  [MGI Ref ID J:177029]