We have applied crosslinking-mass spectrometry on exogenously expressed, purified dysferlin. The results we obtained strongly indicated, as supported by results from other groups and by recent AlphaFold predictions, that while the domains making up this dysferlin appear correctly folded, in the absence of its normal interacting partners dysferlin assumes a “beads on a string” flexible conformation. Hence, we needed to develop tools to examine dysferlin and its interactors from a native environment. To generate reagents that could capture dysferlin when released from a native cell environment, we made nanobodies from camelids (llamas), the smallest monoclonal antibodies that can be made and that have numerous advantages in terms of stability and ease of manufacture and use. We successfully immunized llamas and raised a strong response against human dysferlin. These animals represent an ongoing resource for re-screening for new nanobodies, and as a source of polyclonal sera. From them, we isolated four lead well-performing anti-dysferlin nanobodies, we term LaD17, LaD20, LaD35, and LaD40. Each specifically recognizes dysferlin by immunoblotting and immunofluorescence microscopy. These have also proven to be extremely potent affinity capture reagents, able to purify native dysferlin and its interacting partners ex vivo. We therefore optimized our cell cryomilling, solubilization, and macromolecular complex stabilization methodologies around these nanobodies, allowing us to isolate our first full dysferlin interactomes from unwounded (resting) myotube (muscle proxy) cells. Importantly, our methodology now includes an ultrafast cell freeze and harvest method that will also permit preservation of cells immediately after induction of wounding. This preliminary interactome proved to be rich, and as a key point contained known dysferlin-interacting proteins such as myoferlin, desmin, ryanodine receptor, and nestin. We are now actively pursuing these approaches to map the functional interactomes of dysferlin prior to and after wounding / stress.